. 2020 Sep 9;9(9):740. doi: 10.3390/pathogens9090740

Table 2.

Fluorescence intensity of Sytox green of L3s treated with a combined coriander oil-linalool preparation at five different concentrations compared with positive control (dead L3s), negative control (live L3s) and blank (L3s-free).

F.C. (%)	H. contortus	T. axei	T. colubriformis	T. circumcincta	T. vitrinus	C. oncophora	Mixed Infection
2%	100 ^a	100 ^a	100 ^a	100 ^a	100 ^a	76.1 (8.5) ^b	100 ^a
1%	69.2 (13.4) ^a	100 ^a	91.6 (3.33) ^a	100 ^a	88.8 (6.6) ^a	76.1 (4.2) ^b	85.7 (7.1) ^a
0.5%	46.1 (11.5) ^b	88.8 (6.6) ^a	83.3 (8.33) ^a	81.8 (7.2) ^a	55.5 (8.8) ^b	73.9 (2.8) ^b	71.4 (1.43) ^b
0.25%	42.3 (11.5) ^b	77.7 (13.1) ^b	58.3 (5.2) ^b	72.7 (8.1) ^a	55.5 (5.5) ^b	65.2 (4.5) ^b	57.1 (2.49) ^b
0.125%	19.2 (1.9) ^b	44.4 (16.3) ^b	58.3 (1.6) ^b	27.2 (9.1) ^b	22.2 (3.3) ^b	28.5 (4.8) ^b	35.7 (7.4) ^b
IC₅₀ (%)	0.58	0.14	0.104	0.18	0.21	0.20	0.23
R²	0.38	0.17	0.22	0.66	0.12	0.21	0.79
+ve control	92.3 (7.3) ^a	88.7 (5.4) ^a	83.3 (4.6) ^a	72.7 (9.1) ^a	88.8 (7.3) ^a	100 ^a	100 ^a
–ve control	34.6 (3.8) ^b	22.2 (5.7) ^b	33.3 (8.3) ^b	27.2 (7.2) ^b	33.3 (3.4) ^b	32.4 (2.7) ^b	34.6 (1.3) ^b

Data are presented as the normalized mean of fluorescence intensity (± SEM) and show the effect of treatment with coriander oil-linalool combination on the fluorescence uptake of Sytox green by dead L3s. ^a No statistical difference between positive control and treatments (p > 0.05). ^b Significant difference between positive control and treatments (p < 0.05; ANOVA followed by Tukey test). Abbreviations: F.C. = Final concentration; +ve control (positive control; L3s treated with 50% methanol); –ve control (negative control; L3s treated with PBS-Tween 2%); IC₅₀ = inhibitory concentration 50%; R² = nonlinear correlation.