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. 2020 Sep 9;9(9):740. doi: 10.3390/pathogens9090740

Table 3.

Fluorescence intensity of propidium iodide of L3s treated with a combined coriander oil-linalool preparation at five different concentrations compared with positive control (dead L3s), negative control (live L3s) and blank (L3s-free).

F.C. (%) H. contortus T. axei T. colubriformis T. circumcincta T. vitrinus C. oncophora Mixed Infection
2% 100 a 100 a 100 a 100 a 100 a 78.26 (5.9) b 100 a
1% 56.1 (6.8) b 76.4 (4.7) a 76.3 (3.8) a 100 a 84.6 (3.3) a 72.46 (3.3) b 73.0 (8.4) a
0.5% 39 (5.6) b 58.8 (7.5) b 34.2 (13.8) b 40 (9.2) b 46.1 (8.5) b 69.5 (3.3) b 42.3 (5.1) b
0.25% 39 (10.8) b 58.8 (2.9) b 31.5 (9.05) b 40 (8.8) b 34.6 (8.5) b 55.1 (8.8) b 34.6 (5.1) b
0.125% 2.4 (0.1) b 41.1 (4.5) b 15.7 (5.8) b 6.6 (1.9) b 30.7 (1.4) b 50.7 (7.3) b 11.5 (6.7) b
IC50 (%) 0.58 0.25 0.52 0.41 0.38 0.42 0.49
R2 0.37 0.07 0.35 0.12 0.14 0.09 0.75
+ve control 69.8 (3.2) a 97.7 (1.17) a 80.9 (8.5) a 94.2 (2.6) a 88.8 (4.07) a 100 a 89.8 (6.7) a
–ve control 50.6 (4.3) b 62.2 (7.5) b 39.6 (3.1) b 71.1 (3.4) b 51.8 (1.8) b 50.7 (7.2) b 55.9 (5.1) b

Data are presented as the normalized mean of fluorescence intensity (± SEM) and show the effect of exposure to coriander oil-linalool combination on the fluorescence uptake of propidium iodide by dead L3s. a Non-statistical difference between positive control and treatments (p > 0.05) b Significant difference between positive control and treatments (p < 0.05; ANOVA followed by Tukey test) Abbreviations: F.C. = Final concentration; +ve control (positive control; L3s treated with 50% methanol); –ve control (negative control; L3s treated with PBS-Tween 2%); IC50 = inhibitory concentration 50%; R2 = nonlinear correlation.