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. 2020 Sep 9;9(9):740. doi: 10.3390/pathogens9090740

Table 4.

Fluorescence intensity of C12-resazurin of L3s treated with a combined coriander-linalool preparation at five different concentrations compared with positive control (dead L3s), negative control (live L3s) and blank (L3s-free).

F.C. (%) H. contortus T. axei T. colubriformis T. circumcincta T. vitrinus C. oncophora Mixed Infection
2% 0.00 a 0.00 a 0.00 a 0.00 a 0.00 a 30.7 (4.16) a 0.00 a
1% 23.1 (3.2) a 40 (5.7) a 23.1 (7.6) a 53.8 (1.5) b 25 (2.0) a 100 b 40 (6.8) b
0.5% 46.1 (4.52) b 60 (1.1) b 69.2 (2.8) b 69.2 (7.5) b 80 (4.0) b 53.8 (6.2) b 66.6 (4.5) b
0.25% 69.2 (3.9) b 70 (3.6) b 100 b 90 (2.5) b 90 (6.1) b 76.9 (8.3) b 80 (4.5) b
0.125% 100 b 100 b 100 b 100 b 100 b 76.9 (12.5) b 100 b
IC50 (%) 0.31 0.36 0.66 0.56 0.72 0.15 0.51
R2 0.05 0.01 0.18 0.03 0.24 0.09 0.17
+ve control 30.7 (1.32) a 52.6 (1.52) a 48.7 (2.5) a 30.7 (2) a 30 (2.04) a 45.8 (2.0) a 26.6 (6.6) a
–ve control 66.0 (3.7) b 78.9 (5.2) b 79.4 (5.3) b 77.5 (5) b 71.4 (4.0) b 72.9 (2.0) b 75 (2.2) b

Data are presented as the normalized mean of fluorescence intensity (± SEM) and show the effect of coriander oil-linalool combination on the fluorescence uptake of C12-resazurin by live L3s. a Non-statistical difference between positive control and treatments (p > 0.05) b Significant difference between positive control and treatments (p < 0.05; ANOVA followed by Tukey test). Abbreviations: F.C. = Final concentration; +ve control (positive control; L3s treated with 50% methanol); –ve control (negative control; L3s treated with PBS-Tween 2%); IC50 = inhibitory concentration 50%; R2 = nonlinear correlation.