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. 2020 Sep 22;56(9):484. doi: 10.3390/medicina56090484

Figure 3.

Figure 3

Analyses of adipose tissue membranes for C5L2 and CD36. ELISA for C5L2 in adipose tissue membrane lysates. Fat membrane lysates were prepared from epidydimal fat pads of wild type mice fed on a normal diet (WT ND) and of wild type and properdin knockout mice fed on a high fat diet (WT HFD, KO HFD) for 12 weeks and C5L2 content measured by ELISA. Statistical analysis was performed using the Mann-Whitney U test. Data expressed as mean and standard deviation. ** p < 0.01 (a). Western blot of adipose tissue membrane lysates for CD36. top panel, Western blot for CD36 in adipose tissue membrane lysates from C57BL/6J wild type (WT) and properdin knockout mice (KO) fed a high-fat diet (HFD) for 12 weeks and a wild type control mouse fed on normal chow. Note, the predicted molecular weight is 53 kDa, however its observed weight (when separated on an SDS PAGE gel) tends to be around 78–94 kDa [56,57] due to the post translational addition of glycans which are necessary for its translocation to the plasma membrane [58]. Bottom panel, Ponceau stain of the membrane immediately following transfer (b).