Table 4.
Sequence, amplicon size and annealing temperature of oligos used in the study.
| Oligo | Sequence (5′→3′) | Amplicon Size (bp) | Annealing Tmp (°C) | Reference |
|---|---|---|---|---|
| (16-23S ITS) P-1512F | GTCGTAACAAGGTAGCCGTA | 607 | 60 | [33] |
| (16-23S ITS) P-6R | GGGTTC/TCCCCA/GTTCRGAAAT | |||
| blaOXA-69F (F) | CTAATAATTGATCTACTCAAG | 975 | 48 | [60] |
| blaOXA-69R (R) | CCAGTGGATGGATGGATAGATTATC | |||
| blaOXA-51F (F) | TAATGCTTTGATCGGCCTTG | 353 | 52 | [63] |
| blaOXA-51R (R) | TGGATTGCACTTCATCTTGG | |||
| blaOXA-23F (F) | GATGTGTCATAGTATTCGTCGT | 1058 | [64] | |
| blaOXA-23R (R) | TCACAACAACTAAAAGCACTGT | |||
| blaOXA-24F (F) | GGTTAGTTGGCCCCCTAAAA | 246 | [63] | |
| blaOXA-24R (R) | AGTTGAGCGAAAAGGGGATT | |||
| blaOXA-58F (F) | AAGTATTGGGGCTTGTGCTG | 599 | [63] | |
| blaOXA-58R (R) | CCCCTCTGCGCTCTACATAC | |||
| (blaIMP) IMP-F (blaIMP) IMP-R |
GGAATAGAGTGGCTTAAYTCTC GGTTTAAYAAAACAACCACC |
232 | 52 | [63] |
| (blaVIM) VIM-F (blaVIM) VIM-R |
GATGGTGTTTGGTCGCATA CGAATGCGCAGCACCAG |
390 | ||
| (blaNDM) NDM-F (blaNDM) NDM-R |
GGTTTGGCGATCTGGTTTTC CGGAATGGCTCATCACGATC |
621 | ||
| (blaKPC) KPC-F (blaKPC) KPC-R |
CGTCTAGTTCTGCTGTCTTG CTTGTCATCCTTGTTAGGCG |
798 | ||
| (blaGES) GES-F (blaGES) GES-R |
ATGCGCTTCATTCACGCAC CTATTTGTCCGTGCTCAGGA |
863 | 55 | [65] |
| OPM-01 (P1) | GTTGGTGGCT | Variable | 40 | Eurofins Kit |
| OPM-07 (P2) | CCGTGACTCA | |||
| OPM-09 (P3) | GTCTTGCGGA | |||
| OPQ-06 (P4) | GAGCGCCTTG | |||
| OPQ-08 (P5) | CTCCAGCGGA | |||
| OPM-03 (P6) | GGGGGATGAG | |||
| OPQ-02 (P7) | TCTGTCGGTC | |||
| OPM-05 (P8) | GGGAACGTGT | |||
| OPQ-04 (P9) | AGTGCGCTGA | |||
| OPQ-10 (P10) | TGTGCCCGAA |