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Susceptibility of proteins to enzymatic digestion. The panels represent: (A) SDS–PAGE and (B) Western Blot of duplicate gel. The lanes represent: MW, molecular weight standard (numbers on left in kD); 1, whole cell lysate (WCE) treated with trypsin for 24 h; 2, WCE untreated; 3, WCE treated with proteinase K for 24 h; 4, SVs treated with trypsin for 24 h; 5, SVs untreated; 6, SVs treated with proteinase K.
