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Cytotoxicity of CVs and components. Aliquots of vesicles or their components were incubated with THP-1 cells for 24 h and the viability assessed using an Alamar Blue assay from Biorad. 1% sodium azide was used as a killing control. The data were normalized to 100% of the reading obtained in the assay with untreated cells. All data points were assayed in triplicate. Error bars represent one standard deviation.
