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. 2020 Oct 14;20:998. doi: 10.1186/s12885-020-07394-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — USP6NL exerts pro-oncogenic functions in TNBC by affecting cell proliferation, apoptosis, migration, invasion and EMT. a. The expression of USP6NL in TNBC cell lines and human normal breast cell line MCF-10A were respectively assessed with RT-qPCR. b. The RT-qPCR analyzed the interference efficiency of USP6NL in both cells transfected with sh-USP6NL#1/2. c. Colony formation assay was conducted to measure the change of cell proliferation in cells with or without USP6NL inhibition. d. Flow cytometry analysis determined the apoptosis rate of USP6NL-silenced cells. The X axis of the FACS dot plots meant cells labelled with FITC and the Y axis indicated cells dyed by PI. e-f. JC-1 assay and TUNEL assay were adopted to examine cell apoptosis under USP6NL interference or not. g-h. Transwell assay was used to assess the migration and invasion of TNBC cells with silenced USP6NL or not. i. EMT process in TNBC cells was also examined by IF analysis of E-cadherin and N-cadherin staining. *P < 0.05, **P < 0.01