Fig. 2.

MiR-142-3p acts as the upstream of USP6NL in TNBC. a. Bioinformatics tools were used to predict candidate miRNAs binding to USP6NL. b. The expression of miR-142-3p/miR-200c-3p was examined by RT-qPCR in TNBC cells and normal MCF-10A cells. c-d. RT-qPCR detected miR-142-3p and USP6NL expression in TNBC cells transfected with miR-142-3p mimics. e-f. RIP assay and RNA pull down assay were carried out to verify the interplay between miR-142-3p and USP6NL. g. The binding sites of miR-142-3p to USP6NL’UTR were predicted by ENCORI. h. Luciferase reporter assay was applied to confirm the connection between miR-142-3p and USP6NL. *P < 0.05, **P < 0.01. n.s.: no significance