Figure 1.

The concentrations of malondialdehyde + 4-hydroxyalkenals (MDA+4-HDA) in porcine ovary and thyroid homogenates. Homogenates were incubated in the presence of FeSO₄ (2400, 120, 600, 300, and 150 µM) plus H₂O₂ (5 mM) used to induce lipid peroxidation and, additionally, in the presence of 17β-estradiol (1 mM; 100, 10.0, 1.0 µM; 100, 10.0, 1.0 nM; 100, 10.0, 1.0 pM). Only four concentrations of 17β-estradiol are presented in Figure 1, as other concentrations revealed exactly the same effects as the concentration of 1 µM. Data are expressed as the amount of MDA+4-HDA (nmol) per mg of protein. Bars represent the mean ± SE of three independent experiments run in duplicates. ^a p < 0.05 vs. control ‘0’ in the same tissue; ^b p < 0.05 vs. respective concentration of Fe²⁺ (+H₂O₂) without 17β-estradiol; ^c p < 0.05 vs. respective concentration of Fe²⁺ (+H₂O₂) + 17β-estradiol 10 µM; * p < 0.05 vs. the same substance/concentration in the other tissue.