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. 2020 Sep 11;9(9):596. doi: 10.3390/antibiotics9090596

Table 4.

Plasmid and primers used for PCR experiments.

Plasmid pBBR1MCS-5 4768 bp Gentamycin [48]
DNA Synthesis Primers Used in this Study
PA1113 gene synthesis Fw 5′-ATGATGGCCATATCCGGGGAC-3′
Rev 5′-ACATCTAGATGAGCGTCAAGTACC-3′
367bp amplicon Fw 5′-TGCCAGCCATTCTCTCCTC-3′
Rev 5′-CGGAGCTACCGTTGGTTTC-3′
317 bp amplicon Fw 5′-CGTTCGTCTTCTACAGCCTG-3′
Rev 5′-ATCGAACAGGGTCGACTTGC-3′
1171 bp amplicon Fw 5′-TGCCAGCCATTCTCTCCTC-3′
Rev 5′-ATCGAACAGGGTCGACTTGC-3′
16S RNA Fw 5′-AAGCAACGCGAAGAACCTTA-3′
Rev 5′-CACCGGCAGTCTCCTTAGAG-3′
AprA (alcaline protease) Fw 5′-TACGGCTTCAACTCCAACAC-3′
Rev 5′-TCGACGTATTGCAGCACCA-3′
rpsL (RNA pol sub-unit) Fw 5′-GCAACTATCAACCAGCTGGTG-3′
Rev 5′-GCTGTGCTCTTGCAGGTTGTG-3′