PA1113 gene synthesis |
Fw 5′-ATGATGGCCATATCCGGGGAC-3′ |
Rev 5′-ACATCTAGATGAGCGTCAAGTACC-3′ |
367bp amplicon |
Fw 5′-TGCCAGCCATTCTCTCCTC-3′ |
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Rev 5′-CGGAGCTACCGTTGGTTTC-3′ |
317 bp amplicon |
Fw 5′-CGTTCGTCTTCTACAGCCTG-3′ |
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Rev 5′-ATCGAACAGGGTCGACTTGC-3′ |
1171 bp amplicon |
Fw 5′-TGCCAGCCATTCTCTCCTC-3′ |
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Rev 5′-ATCGAACAGGGTCGACTTGC-3′ |
16S RNA |
Fw 5′-AAGCAACGCGAAGAACCTTA-3′ |
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Rev 5′-CACCGGCAGTCTCCTTAGAG-3′ |
AprA (alcaline protease) |
Fw 5′-TACGGCTTCAACTCCAACAC-3′ |
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Rev 5′-TCGACGTATTGCAGCACCA-3′ |
rpsL (RNA pol sub-unit) |
Fw 5′-GCAACTATCAACCAGCTGGTG-3′ |
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Rev 5′-GCTGTGCTCTTGCAGGTTGTG-3′ |