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. 2020 Sep 17;9(9):758. doi: 10.3390/pathogens9090758

Figure 2.

Figure 2

SDS–PAGE analysis of recombinant FgTPx (rFgTPx). Lane M: protein molecular weight marker in kDa; Lane NC: bacterial cell lysate without induction; Lane 1: cell lysate with induction for 16 h at 15 °C; Lane 2: cell lysate with induction for 4 h at 37 °C; Lane NC1: supernatant of cell lysate without induction; Lane 3: supernatant of cell lysate with induction for 16 h at 15 °C; Lane 4: supernatant of cell lysate with induction for 4 h at 37 °C; Lane NC2: pellet of cell lysate without induction; Lane 5: pellet of cell lysate with induction for 16 h at 15 °C; Lane 6: pellet of cell lysate with induction for 4 h at 37 °C. The red arrows denote the recombinant protein (rFgTPx).