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. 2020 Sep 17;9(9):758. doi: 10.3390/pathogens9090758

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The rFgTPx induced apoptosis in goat PBMCs. Apoptotic cells were determined by annexin V/PI staining and flow cytometry. (A) Flow cytometric analysis shows the death of goat PBMCs in response to exposure to increasing concentrations of rFgTPx. (B) The percentage of apoptotic cells (annexin V+/PI–) compared to total cell population. Graphs represent means ± standard deviations of data from three independent experiments. The asterisks indicate statistically significant differences between treated and untreated control PBMCs (**** p < 0.0001; ns: non-significant).