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. 2020 Oct 15;17:86. doi: 10.1186/s12986-020-00508-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Molecular assessment of Klotho-α and FGF23 in the hippocampus and prefrontal cortex of experimental groups. Rats treated with high-fat, high-protein, low-calorie, low-calorie high-protein diets for 10 weeks. Control adult and old rats treated with rodent standard pellet. After behavioral tests, rats were euthanized and brains of three of them perfused for histological evaluation in each group (n = 3) and brains of other three rats in each groups were collected for Western blotting technique (n = 4). Immunoflorence assay a showed the Klotho-α distribution in the hippocampus and prefrontal cortex. Mean of fluorescence intensity showed Klotho-α positive cell in the CA1 and prefrontal cortex (b). A represented blot showed Klotho-α and FGF23 protein level (c) and the density of Klotho-α bands (d) and FGF23 band (e) were measured in the hippocampus and prefrontal cortex (n = 4 and technical repeat for each n is 3). Data were presented as Mean ± S.D. ***P < 0.001 ver. Adult-C, #P < 0.05, ##P < 0.01, ###P < 0.001 ver. Old-C. $P < 0.05 ver. High-protein. Adult-C adult control rats, Old-C old control rats