Figure 5.
Confocal images of GFP-HUVECs co-cultured with hASCs and stained for α-SMA. Constructs containing hCOL (a,e), hCOL/Agarose (b,g), MatrigelTM/rCOL (c,f), and MatrigelTM/rCOL/Agarose (d,h) were analyzed after 14 days of cultivation. (a–d) Confocal images displaying an overview in a low magnification. (e–h) Virtual stacks of high magnification images with respective orthogonal views. α-SMA positive cells wrap around GFP-HUVEC cords under all conditions. Scale bar: (a–d): 200 µm, (e–h): 50 µm.