Spinal cord histopathology in CD20dbtg mice treated from day 30 after the EAE peak with OBZ or huIgG1, respectively. (A) Representative light microscopic images of the spinal cord. The lesion area can be seen to the right of the dashed line. The scale bar represents 75 µm. (B) Representative electron micrographs of the spinal cord. The scale bar represents 5 µm. A diamond marks an exemplary demyelinating axon and a star an axolytic axon, respectively. Images of untreated non-immunised mice have been added in both (A) and (B) for reference. (C) Quantification of the treatment effect on lesion area, g-ratio, demyelination, remyelination, axolysis and number of axons. Values are displayed as mean values ± standard error of the mean (SEM). *: p < 0.05; **: p < 0.01. Statistical significance was determined using the Mann–Whitney U test.