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. 2020 Aug 26;12(9):810. doi: 10.3390/pharmaceutics12090810

Figure 1.

Figure 1

Native mass spectrometry of intact proteins. (A) UV size exclusion chromatograms FabA, HSA and FabA–HSA complexes formed after 4 h incubation with human VH supplemented with albumin (at 1:1 and 1:5 ratio). (B) Deconvoluted intact mass spectrum of chromatographic peak 1 corresponding to a molecular weight of FabA and (C) HSA. (D) Deconvoluted spectra of two mass ranges. The lower mass range contains FabA and HSA, while the higher mass range covers the equimolar FabA–HSA complex of 126 kDa. (E) Deconvoluted intact mass spectra of three mass ranges, with FabA in the lowest, FabA–HSA and dimeric (HSA)2 in the middle, and two high molecular mass complexes of 186 and 193 kDa in the highest.