Table 2.
Experiment | FabA Dose | 3H-FabA Concentration 1 | Albumin Concentration 2 | ||
---|---|---|---|---|---|
µM | mg/L | µM | mg/L | ||
Formulation of in vivo rabbit study | 16.8 | 1000 | 20.0 | 1328 | |
16.8 | 1000 | 300 | 19,920 | ||
In vivo rabbit VH | Low dose 3 | 0.57 | 33 | 0.67 | 44 |
0.57 | 33 | 10.0 | 664 | ||
In vitro cynomolgus monkey VH | Low dose | 0.57 | 33 | 0.53 | 35 |
0.57 | 33 | 50.0 | 3320 | ||
0.57 | 33 | 100 | 6640 | ||
0.57 | 33 | 250 | 16,600 | ||
High dose 4 | 5.7 | 330 | 0.53 | 35 | |
5.7 | 330 | 50.0 | 3320 | ||
5.7 | 330 | 100 | 6640 | ||
5.7 | 330 | 250 | 16,600 | ||
In vitro human VH (no eye disease) | Low dose | 0.57 | 33 | 4.22 | 280 |
0.57 | 33 | 50.0 | 3320 | ||
0.57 | 33 | 100 | 6640 | ||
0.57 | 33 | 250 | 16,600 | ||
High dose 4 | 5.7 | 330 | 4.22 | 280 | |
5.7 | 330 | 50.0 | 3320 | ||
5.7 | 330 | 100 | 6640 | ||
5.7 | 330 | 250 | 16,600 | ||
In vitro human VH (PDR) | Low dose | 0.57 | 33 | 10.53 | 699 |
0.57 | 33 | 50.0 | 3320 |
1 Calculated using FabA MW of 59.5 kDa. 2 Calculated using albumin MW of 66.4 kDa. 3 Calculated assuming a rabbit vitreous humor volume of 1.5 mL [13]. 4 10-fold of low dose (0.57 µM 3H-FabA complemented with 5.13 µM unlabeled FabA).