Figure 4.

The cocultivation of S. aureus with rLys P. pastoris results in S. aureus killing. (A) Viability of S. aureus in coculture with 10⁷ CFU/mL of rLys P. pastoris (red) or control mCherry-producing P. pastoris (gray). Dashed dots indicate that no viable S. aureus was detected at the respective time point. The detection limit of viable S. aureus cells was ∼10³ CFU/mL. (B) Viability of S. aureus in coculture with 10⁶ CFU/mL of rLys P. pastoris at various S. aureus loads. 10¹⁰ (aquamarine), 10⁸ (green) and 10⁶ (orange) CFU/mL of S. aureus were used as a starting point. Data represent the mean of three biological replicates ± SD. (C,D) illustrate representative images of S. aureus/P. pastoris cocultures at various time points, corresponding to panels (A,B), respectively.