Figure 5.

Zinc induces almost immediate ATP efflux. C. albicans SC5314 bioluminescent ATP efflux assay. Cells were treated at 30 °C with 0.88 fmol/ cell. Hst 5 (red), Hst 5ΔMB (blue), or P113 (green) in 10 mM sodium phosphate buffer pH 7.4 with or without added Zn²⁺ at a one to two peptides ratio (hatched). Cells were removed from samples via centrifugation and aliquots of supernatant were taken at 1 min and 10 min time points to measure extracellular ATP by luminescence. Experiments were repeated on at least three separate days and averaged. Significance of differences in ATP efflux calculated using one way ANOVA with Sidak’s multiple comparison test. ** indicates p ≤ 0.01, *** indicates p ≤ 0.001.