Table 8.

Set of primers specific for Leishmania braziliensis targeting the IGS rRNA region.

Genome location	Primers	Size	% C/G	T m
IGS rRNA	Lb/IGS F 5′-CGCACAAAACACACACTGGT-3′	20 bases	50%	59.8°C
IGS rRNA	Lb/IGS R 5′-TTCATGCTCAGGGACACACTC-3′	21 bases	52%	60°C

Legend: IGS—Intergenic Spacer; rRNA—ribosomal RNA; Lb—Leishmania braziliensis; F—forward; R—reverse; % C/G—percentage of cytosine/guanine; T_m—melting temperature.