. 2020 Jun 30;7(3):80. doi: 10.3390/vetsci7030080

Table 3.

Mortality, total and mean scoring for macroscopic lesions per organ in the O78, O18 and O111 challenge trials. Macroscopic lesion scores are expressed for each organ by mean ± standard deviation.

Challenge Strain	O78				O18				O111
Vaccination protocol	A	B	C	D	A	B	C	D	A	B	C	D
Mortality (%)	9/20^a (45%)	7/20^a (35%)	0/20^b (0%)	0/20^b (0%)	0/20 (0%)	1/20 (5%)	1/20 (5%)	0/20 (0%)	2/20 (10%)	0/20 (0%)	0/20 (0%)	0/20 (0%)
Heart	2^a ± 0.72	2^a ± 0.85	1^b ± 0.45	1.05^b ± 0.51	1.5^a ± 0.76	1.65^ab ± 1.03	1.55^ab ± 0.99	0.8^b ± 1.00	1.6^a ± 0.99	1.55^a ± 0.51	0.55^b ± 0.51	0.55^b ± 0.51
Liver	1.3^a ± 0.80	1.1^a ± 0.78	0^b ± 0.00	0.1^b ± 0.44	0.55 ± 0.82	0.9 ± 0.91	0.9 ± 1.02	0.35 ± 0.67	0.95^a ± 0.82	0.6^a ± 0.68	0^b ± 0.00	0.1^b ± 0.30
Spleen	1 ± 0.00	0.95 ± 0.22	1 ± 0.00	1 ± 0.00	0.95^a ± 0.22	0.8^ab ± 0.41	0.9^a ± 0.30	0.5^b ± 0.51	1^a ± 0.00	1^a ± 0.00	0.6^b ± 0.50	0.6^b ± 0.50
Air sacs	1.75^ab ± 0.78	2^a ± 0.72	1.2^b ± 0.61	1.25^b ± 0.85	1.75^a ± 0.78	1.55^a ± 0.75	1.6^a ± 0.94	0.55^b ± 0.75	1.4^a ± 0.59	1.6^a ± 0.50	0.75^b ± 0.55	0.6^b ± 0.50
Lungs	2.15^a ± 1.42	1.6^ab ± 1.23	1^b ± 0.79	1.3^ab ± 0.80	1.6 ± 0.75	1.2 ± 0.89	1.1 ± 1.02	1.1 ± 1.16	1.3 ± 1.38	0.7 ± 0.73	0.9 ± 0.85	0.65 ± 0.74
Peritonitis	1.1^a ± 0.55	1^a ± 0.64	0.2^b ± 041	0.4^b ± 0.50	0.6^ab ± 0.82	0.95^a ± 0.88	0.95^a ± 0.88	0.2^b ± 0.52	0.95^a ± 0.68	0.8^a ± 0.52	0^b ± 0.00	0.1^b ± 0.30
Total	9.3^a ± 2.57	8.65^a ± 3.24	4.4^b ± 1.35	5.1^b ± 2.04	6.95^a ± 2.99	7.05^a ± 3.96	7^a ± 4.02	3.5^b ± 3.00	7.2^a ± 2.74	6.25^a ± 1.80	2.8^b ± 1.36	2.6^b ± 1.72
Total w/o spleen*	8.3^a ± 2.57	7.7^a ± 3.14	3.4^b ± 1.35	4.1^b ± 2.04	6^a ± 2.97	6.25^a ± 3.72	6.1^a ± 4.87	3^b ± 2.86	6.2^a ± 2.74	5.25^a ± 1.80	2.2^b ± 1.28	2^b ± 1.41

* The lesion score of spleen has been subtracted. No results are reported for the non-vaccinated non-challenged group (negative control) due to a total lack of mortality and macroscopic lesion scoring for each euthanized bird. a,b: values without superscript do not differ significantly (p > 0.05), while different superscripts in the same row correspond to statistically significant differences between groups (p < 0.05).