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Determination of binding affinity of anti-PODXL antibodies (PcMab-60 and 60-mG2a-f) using flow cytometry. MIA PaCa-2 cells were suspended in 100 μL of serially diluted antibodies (6 ng/mL to 100 μg/mL), and secondary antibodies were then added. Fluorescence data were collected using a cell analyzer. GeoMean, the geometric mean of fluorescence intensity.
