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(A) SDS-Page silver staining of yPDE2 purification (enzyme MW ≈63 KDa); (B) Lineweaver–Burk plot of the effect of different RSV concentrations on yPDE2 activity (sample buffer at 25 °C); (C) competitive Michaelis–Menten plot (Equation (3)); (D) effect of different inhibitors against yPDE2 activity (sample buffer at 25 °C) using 0.08 μM of enzyme in all cases.
