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. 2020 Aug 19;7(3):115. doi: 10.3390/vetsci7030115

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Adherent cells differentiated to KUL01⁺ CSF1R⁺ MHC-II⁺ macrophages (Mph) after 7 days of culture. (A) Adherent cells were characterized after 24 h of culture by flow cytometry. (B) Adherent unstimulated cells were characterized after 7 days of culture by flow cytometry. The cells were selected for viability (7-AAD⁻), forward and side light scatter (FSC vs. SSC), and assessed for the expression of KUL01, CSF1R, MHC-II and CD40. The histograms show expression of the macrophage markers in blue and fluorescent-minus-one (FMO) staining controls in grey. (C) The percentages of macrophages from day 1 and day 7.