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Primary stimulation with LPS resulted in enhanced nitric oxide production. Fresh isolated adherent cells were primary stimulated with culture medium (UNSTIM), LPS (10 μg/mL), M-βG (10 μg/mL), M-βG + IL-4 (10 μg/mL + 100 ng/mL) or IL-4 (100 ng/mL). LPS induced NO production (N = 21 chickens; N IL-4 = 5 chickens). Each bar represents mean ± SEM. *** p < 0.001.
