Figure A3.

Bone marrow-derived adherent cells primary stimulated with M-βG + IL-4 failed to enhance NO production after secondary stimulation of LPS. Freshly isolated chicken bone marrow-derived adherent cells were primary stimulated with culture medium (UNSTIM), LPS (10 μg/mL), M-βG (10 μg/mL), M-βG + IL-4 (10 μg/mL + 100 ng/mL) or IL-4 (100 ng/mL), on D0. The cells were secondary stimulated with LPS (10 μg/mL) except the negative control (UNSTIM-UNSTIM) on D6. Results after this LPS stimulation are shown in this figure. LPS induced NO production for all treatment groups. In contrast to blood-derived macrophages, a secondary stimulation with LPS did not affect the NO production in M-βG + IL-4 primary stimulated cells compared to the primary unstimulated cells (UNSTIM-LPS). (N = 6–10 chickens; N for UNSTIM-UNSTIM and IL-4 = 4 chickens). Each bar represents mean ± SEM.