Skip to main content
. 2020 Sep 17;21(18):6834. doi: 10.3390/ijms21186834

Figure 6.

Figure 6

Intracisternal injection of C1q causes mechanical hypersensitivity and astrocytic activation. (A) Time-course of mechanical head withdrawal threshold (MHWT) in the whisker pad skin. PBS: n = 5, C1q: n = 5, two-way ANOVA post hoc Bonferroni test, *** p < 0.001 (vs. PBS-treated group). (B,D,F) Representative images of c-Fos (B), GFAP (D), and Iba1 (F) immunofluorescence in the Vc 7 days after PBS or C1q administration. Arrowheads indicate c-Fos-IR cells. Insets indicate enlarged images of the region indicated in the open square. (C,E,G) Averaged number of c-Fos-IR cells (C) and fluorescence intensity (FI) of GFAP (E) and Iba1 (G) in the Vc on day 7 of PBS or C1q-administrated rats. PBS: n = 5, C1q: n = 5, unpaired Student’s t-test, *** p < 0.001. n.s.: not significant. The data represent the means ± SEM.