Skip to main content
. 2020 Aug 20;39(20):e103667. doi: 10.15252/embj.2019103667

Figure 1. Establishment of FANS for stem cells of the shoot apical meristem (SAM).

Figure 1

  1. Expression of H2B‐mCherry under control of the CLV3 promoter in 14‐day-old seedlings. Whole‐mount immunostaining using α‐mCherry antibodies and laser scanning microscopy (scale bar 10 μm).
  2. Example of a FANS experiment: mCherry‐positive (+) and mCherry‐negative (−) gates of DAPI‐gated nuclei. Numbers indicate total number and percent of DAPI (for −) and mCherry (for +) events.
  3. A representative example for enrichment of CLV3 transcript in mCherry‐positive nuclei determined by qRT–PCR and normalized to wt (N = 1).