Figure EV1. Isolation of stem cell nuclei and RNA comparison.

1. Gating strategy used for FANS of stem cells. Representative FANS plots are shown. Events are gated for DAPI (top row) and next either for mCherry⁺ or mCherry⁻ (bottom row). For numbers, see also Appendix Table S1. Reprinted with permission from Gutzat and Mittelsten Scheid (2020).
2. Examples of mCherry‐positive nuclei after FANS (scale bar 5 μm).