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A–C
Representative (n = 3) WB analysis demonstrating that while GAPDH‐HA control and EB1‐Flag do not bind ((A), upper panels and (B), respectively) FEZ1‐Flag control and Flag‐CLIP170 ((A), lower panels and (C), respectively) pelleted with in vitro assembled HIV‐1 CA-NC complexes.
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D
Quantification of the levels of bound CLIP170 as a ratio of total protein across experimental replicates (n = 3) from (C). Statistical significance was determined by t‐test (mean ± SEM) ***P < 0.001.
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E
Representative WB analysis (n = 3) showing the levels of HIV‐1 p24 CA in mock and virus fractions separated by sucrose gradient centrifugation. Fractions 1‐3 contain free p24 not associated with the intact cores, while fractions #8 and # 9 harboring intact HIV‐1 cores.
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F
Flag‐CLIP170, but not Flag control, also binds to fractions containing intact HIV‐1 cores (viruses #8 and #9) but not with Mock fractions.
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G
HIV-1‐WT-luc infection is increased in CHME3 cells stably expressing Flag‐CLIP170, but not control Flag or Flag‐CLIP170ΔCAP‐gly. Statistical significance was determined by one‐way ANOVA. *P < 0.05. Mean ± SEM from three independent experiments is shown.
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H
Representative images (n = 3) of CHME3 expressing Flag‐CLIP170 or Flag‐CLIP170ΔCAP‐gly stained for Flag and Tyr‐MTs. Scale bar, 10 μm.
Data information: Molecular weight markers (in kDa) are shown to the right of WBs.
