Figure EV4. The MHR of HIV‐1 capsid encodes an EB1‐mimetic motif.

• A
  EB1 mimetic residues within the MHR of HIV‐1 capsid display a significant degree of conservation across different HIV‐1 strains. Sequence similarities of 88 HIV‐1 MHR from different subtypes obtained from the Los Alamos database. Color scheme indicates hydrophobicity.
• B–D
  An SxIP mutant CLIP170 is impaired in its ability to promote viral translocation to the nucleus. 293A cells were treated with siRNA targeting the 3′UTR of CLIP170 (CLIP170nc2) to limit the influence of endogenous CLIP170 on assays, followed by transfection with either Flag‐tagged WT (Flag‐CLIP170) or mutant CLIP170 lacking SxIP motif (Flag‐CLIP170ΔSxIP) constructs after 24 h. Cells were then infected with HIV‐1-VSV‐GFP-Vpr. (B) WB analysis using anti‐CLIP170 or anti‐Flag antibodies confirms depletion of endogenous and expression of exogenous CLIP170, respectively. (C) Representative staining images for tyrosinated tubulin (Tyr‐MTs), viral particles (GFP), and the nucleus (Hoescht) are shown. Scale bar, 10 μm. (D) Percentage viral particles within 2 μm of the nucleus at 4 h.p.i, scatterplot with mean of ≥ 80 virus particles quantified in ≥ 16 cells per sample. Statistical significance was determined by t‐test. **P < 0.01.