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. 2020 Aug 27;39(20):e103958. doi: 10.15252/embj.2019103958

Figure EV2. HIV‐1 with Gag protease cleavage mutation induces ISGs in macrophages.

Figure EV2

  1. Infection levels of cells from Fig 2C–E. PMA‐treated THP‐1 shSAMHD1 cells transduced for 48 h with HIV‐1 GFP ΔCA‐SP1 viruses (0.1 U RT/ml or 0.5 U RT/ml).
  2. Infection data from Fig 2G. THP-1‐IFIT-1 cells transduced for 48 h with HIV‐1 GFP ΔCA‐SP1 viruses (0.016–0.2 U RT/ml).
  3. Infection data from Fig 2H. THP-1‐IFIT-1 cells transduced for 48 h with HIV‐1 GFP containing either 0% (WT) or 75% ΔCA‐SP1 mutant in the presence of DMSO vehicle or 2 μM ruxolitinib.
  4. Infection data from Fig 2I. Primary MDM transduced for 48 h with WT HIV‐1 GFP or 75% ΔCA‐SP1 mutant (3 × 108 genomes/ml or 1.5 × 109 genomes/ml).
  5. Infection data from Fig 2J and K. Primary MDM transduced for 48 h with WT HIV‐1 GFP or 75% ΔCA‐SP1 mutant (1.5 × 109 genomes/ml) in the presence of DMSO vehicle or 2 μM ruxolitinib.
  6. Repeat of (E) in a second donor.
Data information: Data are individual measurements (B) or mean ± SD, n = 3 (A, C–F), representative of 2 repeats. Statistical analyses were performed using Student's t‐test, with Welch's correction where appropriate and comparing to the DMSO control. **P < 0.01, ***P < 0.001.