Figure EV4. Innate immune activation is DNA sensing‐dependent.

1. Infection data from Fig 4A. PMA‐treated THP-1‐IFIT-1 shSAMHD1 cells lacking STING or MAVS, or a gRNA control (Ctrl) cell line transduced for 48 h with HIV‐1 GFP 75% ΔCA‐SP1 (0.4 U RT/ml).
2. Infection data from Fig 4C and D. PMA‐treated THP‐1 Dual shSAMHD1 cells lacking cGAS or a matching control (Ctrl) cell line transduced for 48 h with HIV‐1 GFP containing either 0% (WT) or 75% ΔCA‐SP1 (1 × 10¹⁰ and 2 × 10¹⁰ genomes/ml).
3. Infection data from Fig 4E and G. PMA‐treated THP-1‐IFIT-1 shSAMHD1 cells lacking STING, MAVS or matching gRNA control (Ctrl) cell lines transduced for 48 h with DRV‐treated HIV‐1 GFP (1 × 10¹⁰ genomes/ml).
4. Infection data from Fig 4F and H. PMA‐treated THP‐1 Dual shSAMHD1 cells lacking cGAS or matching control (Ctrl) cell lines transduced for 48 h with DRV‐treated HIV‐1 GFP (1 × 10¹⁰ genomes/ml).
5. Infection data from Fig 4I. PMA‐treated THP‐1 Dual shSAMHD1 control cells transduced for 48 h with WT, DRV‐treated (DRV, 12.5 nM) or HIV‐1 GFP 90% ΔCA‐SP1 (1 × 10¹⁰ genomes/ml) in the presence of DMSO vehicle, 2 μM ruxolitinib or 0.5 μg/ml H151.

Data information: Data are mean ± SD, n = 3, representative of 2 (C–E) or 3 (A, B) repeats.