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. 2020 Aug 27;39(20):e103958. doi: 10.15252/embj.2019103958

Figure EV5. PF‐74 treatment induces HIV‐1 to trigger a DNA sensing‐dependent ISG response.

Figure EV5

  1. Infection data from Fig 6F. THP-1‐IFIT-1 cells transduced for 48 h with HIV‐1 GFP (3 U/ml RT) in the presence of DMSO control or PF‐74 (10 μM) and ruxolitinib (Rux, 2 μM) as indicated.
  2. Infection data from Fig 6G. THP‐1 Dual shSAMHD1 cells lacking cGAS or a matching control (Ctrl) cell line transduced for 48 h with HIV‐1 GFP (3 and 6 U/ml) in the presence of DMSO vehicle or PF‐74 (10 μM).
  3. Infection data from Fig 6H. THP-1‐IFIT-1 cells lacking MAVS or a matching gRNA control (Ctrl) cell line transduced for 48 h with HIV‐1 GFP (3 and 6 U/ml) in the presence of DMSO vehicle or PF‐74 (10 μM).
Data information: Data are mean ± SD, n = 2 (B), 3 (A) or 4 (C), representative of 3 repeats.