Figure 6. PF‐74 treatment induces HIV‐1 to trigger a DNA sensing‐dependent ISG response.

• A
  Infection data for THP-1‐IFIT-1 cells transduced for 24 h with HIV‐1 GFP (0.1–3 U/ml RT) in the presence of DMSO vehicle or PF‐74 (10 μM).
• B
  IFIT‐1 reporter activity in supernatant from (A).
• C, D
  ISG qRT‐PCR from monocytic THP‐1-IFIT‐1 cells transduced for 24 h with HIV‐1 GFP (0.25–1 U/ml RT) in the presence of DMSO vehicle or PF‐74 (10 μM).
• E
  CXCL‐10 protein in supernatants of THP-1‐IFIT-1 cells transduced for 24 h with HIV‐1 GFP (3 U/ml) in the presence of DMSO vehicle or PF‐74 (10 μM).
• F
  IFIT‐1 reporter activity from THP-1‐IFIT-1 cells transduced for 24 h with HIV‐1 GFP (3 U/ml RT) in the presence of DMSO vehicle or PF‐74 (10 μM) and ruxolitinib (Rux, 2 μM) as indicated.
• G
  IRF reporter activity from THP‐1 Dual shSAMHD1 cells lacking cGAS or a matching control (Ctrl) cell line transduced for 24 h with HIV‐1 GFP (3 and 6 U/ml) in the presence of DMSO vehicle or PF‐74 (10 μM).
• H
  IFIT‐1 reporter activity from THP-1‐IFIT-1 cells lacking MAVS or a matching gRNA control (Ctrl) cell line transduced for 24 h with HIV‐1 GFP (3 and 6 U/ml) in the presence of DMSO vehicle or PF‐74 (10 μM).

Data information: Data are mean ± SD, n = 2 (C‐E, G), 3 (A, F), 4 (H) or 6 (B), representative of 3 repeats. Statistical analyses were performed using Student's t‐test, with Welch's correction where appropriate. *P < 0.05, **P < 0.01, ***P < 0.001. See also Fig EV5.