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. 2020 Jul 24;39(20):e104467. doi: 10.15252/embj.2020104467

Figure EV2. FXR1 does not drive recruitment of lamina‐associated proteins.

Figure EV2

  • A–H
    HeLa cells were treated with the indicated siRNAs, synchronized by double thymidine block and released for 9 (telophase) and 12 (interphase) h and analysed by immunofluorescence microscopy. The nuclear intensity of Lamin A (B), Lamin B1 (D), Emerin (F) and Lap2β (G) was quantified, and 2,000 cells were analysed (mean ± SD, *P < 0.05; ns, non‐significant; N = 3). The nuclear area was quantified (H), and 3,300 cells were analysed (mean ± SD, **P < 0.01; N = 5).
Data information: Scale bars are 5 μm. Statistical significance was assessed by one‐sample two‐tailed Student's t‐test.