Figure EV4. FXR protein family members localize to NE and regulate cytoplasmic Nups.

• A, B
  HeLa cells (A) and mouse C2C12 myoblasts (B) were analysed by immunofluorescence microscopy for FXR1, FXR1 + 2 and FMRP. The magnified framed regions are shown in the corresponding numbered panels.
• C–E
  HeLa cells were treated with the indicated siRNAs, synchronized by double thymidine block and released for 12 h and analysed by Western blot (SE—short exposure, LE—long exposure) (C) and immunofluorescence microscopy (D, E). The percentage of cells with cytoplasmic nucleoporin granules (D) and irregular nuclei (E) was quantified, and 900 cells were analysed (mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001; N = 3).

Data information: Scale bars are 5 μm. Statistical significance was assessed by one‐way ANOVA test with Dunnett's correction (D, E).Source data are available online for this figure.