Figure 5. Cytoplasmic Nup granules are sensitive to 1,6‐hexanediol.

• A
  HeLa cells stably expressing GFP‐Nup133 were synchronized by double thymidine block, released for 12 h, treated or not with NaAsO₂ to induce stress granule formation and with 1,6‐hexanediol, and analysed by immunofluorescence microscopy. The magnified framed regions are shown in the corresponding numbered panels.
• B, C
  HeLa cells stably expressing GFP‐Nup133 were treated with the indicated siRNAs, synchronized by double thymidine block, released for 12 h, treated with or without 1,6‐hexanediol and analysed by immunofluorescence microscopy. The magnified framed regions are shown in the corresponding numbered panels. The percentage of cells with cytoplasmic GFP‐Nup133 granules was quantified in (C), and 3,100 cells were analysed (mean ± SD, **P < 0.01; ***P < 0.001; ****P < 0.0001; N = 3).

Data information: Scale bars are 5 μm. Statistical significance was assessed by one‐way ANOVA test with Sidak's correction.