Fig. 3.

LPS increases the levels of the BKCa channel mRNA and protein expression. a, b Western blot show BKCa channel protein expression. c The expression of the BKCa channel mRNA was analysed using real-time PCR. The results were normalized to the GAPDH level and shown as relative changes. d The function of BKCa channels was detected using a voltage patch clamp, which was activated by 300-ms voltage clamp steps to + 80 V from − 80 mV and then to − 30 mV. The data are presented as the mean ± S.E.M. from three independent experiments. The value 0, 10, 25, 50, and 100 represent the corresponding LPS concentrations (ng/ml). *p < 0.05 and **p < 0.01 compared with the 0 group