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. 2020 Oct 15;39:218. doi: 10.1186/s13046-020-01730-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — SETDB1 regulates CSF-1 level in glioblastoma cells. a Real-time PCR for mRNA level of genes coding for tumor-associated macrophage (TAMs) recruitment associated cytokines in U87 cells with SETDB1 overexpression. b Real-time PCR for mRNA level of genes coding for tumor-associated macrophage (TAMs) recruitment associated cytokines in U251 cells with SETDB1 overexpression. c Real-time PCR for mRNA level of genes coding for tumor-associated macrophage (TAMs) recruitment associated cytokines in U87 cells with SETDB1 knockdown. d Real-time PCR for mRNA level of genes coding for tumor-associated macrophage (TAMs) recruitment associated cytokines in U251 cells with SETDB1 knockdown. e Enzyme-linked immunosorbent assay (ELISA) analysis of CSF-1 concentration in the supernatants of cultured glioblastoma cells with SETDB1 overexpression. f Enzyme-linked immunosorbent assay (ELISA) analysis of CSF-1 concentration in the supernatants of cultured glioblastoma cells with SETDB1 knockdown. g TCGA database indicated the correlation of CD163 and CSF-1 in glioblastoma tissues. h TCGA database indicated the correlation of SETDB1 and CSF-1 in glioblastoma tissues. Results were expressed as means ± SD of 3 independent experiments. **, P < 0.01