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. 2020 Oct 15;39:218. doi: 10.1186/s13046-020-01730-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — SETDB1 promotes macrophage recruitment and polarization. a Transwell migration assay of macrophage by CM from indicated U87 cells. b Real-time PCR for the expression levels of CD68 and CD163 in macrophages treated with CM from U87 cells as indicated. c Flow cytometry analysis for the expression levels of CD163 in macrophages treated with CM from glioblastoma cells as indicated. d Real-time PCR for the mRNA expression of tumor-associated macrophage (TAM) characteristic cytokines in macrophages treated with CM from glioblastoma cells as indicated. e Enzyme-linked immunosorbent assay (ELISA) for the secretion of tumor-associated macrophage (TAM) characteristic cytokines in macrophages treated with CM from U87 cells as indicated. f Real-time PCR for the mRNA expression of M1-related cytokines in macrophages treated with CM from U87 cells as indicated. g Enzyme-linked immunosorbent assay (ELISA) for the secretion of M1-related cytokines in macrophages treated with CM from U87 cells as indicated. Results were expressed as means ± SD of 3 independent experiments. **, P < 0.01