Fig. 7.

SETDB1 promotes tumor growth in a macrophage-dependent manner. a Western blotting of SETDB1 overexpression efficiency in GL261 cells. b Treatment scheme depicting intraperitoneal application of clodronate liposomes or phosphate-buffered saline (PBS) liposomes in C57BL/6 J mice for 4 weeks. GL261 cells were administered at the end of week 2 and animals were sacrificed 2 weeks later. c Representative immunohistochemistry of F4/80+ cells in sections from tumors obtained from C57BL/6 J mice treated with clodronate liposomes or PBS liposomes. Scale bar: 50 μm. d Tumor volume of vector and SETDB1 overexpression tumors from clodronate-liposome or PBS-liposome-treated mice (n = 6 per group). e Tumor weight of vector and SETDB1 overexpression tumors from clodronate-liposome or PBS-liposome-treated mice (n = 6 per group). g The body weight of mice of indicated groups. h A working model of how SETDB1 regulates TAMs recruitment and tumor growth in glioblastoma