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. 2020 Jun 28;8(3):46. doi: 10.3390/toxics8030046

Figure 1.

Figure 1

Increased cellular and acellular generation by medium-chain triglycerides (MCT), vitamin E acetate (VEA), and counterfeit cartridges. (A) Increased cellular ROS generation by cartridge liquid treatments. BEAS-2B cells were seeded at 15,000/well. The following day, cells were treated with 20 µM DCFH-DA in 1% FBS containing media for 30 min. Cells were then washed and treated with 0.25% (v/v) MCT, 0.025% (w/v) or 50 µM Vitamin E acetate, and counterfeit vape cartridge liquids. Fluorescence (485,535 nm) was read at 1.5, 4 and 6 h post-treatment (n = 4). Control vs. MCT *** p < 0.0001. Control vs. VEA * p < 0.05, Control vs. Cartridge p = 0.2236. (B) Increased acellular generation by MCT, VEA, and counterfeit vape cartridges. Air, 50% (w/v) vitamin E acetate, MCT, and counterfeit vape cartridges were bubbled through dichlorofluorescein diacetate (DCFH-DA) dye with two 70 mL puffs. Fluorescence was measured at 485/535 nm. One-way ANOVA *** p < 0.001 vs. air (n = 2–4).