Figure 6.
Induction of differential cytokine response by MCT, VEA, and CBD/counterfeit vape cartridge aerosol exposure in mouse BALF. Wildtype mice with C57BL/6 background were exposed to MCT, vitamin E acetate 50% (w/v), and counterfeit vape cartridges, 1 puff/min for 1 h for three consecutive days using the Scireq inExpose system. Immediately after the last exposure, the mice were sacrificed and the BALF was collected. Secreted inflammatory mediators were measured using BioRad 23-plex, group 1 Luminex kit. (A) IL-6, (B) eotaxin, and (C) G-CSF were (D) MCP-1, (E) RANTES, (F) IL-17A, (G) IL-12p40, and (H) IL-4 are reported. One-way ANOVA * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. air, p > 0.05 were considered nonsignificant and were not denoted with asterisks (n = 6).