Table 1.
Untargeted lipidomic analysis (DG: diradylglycerols, CE: sterols, PC: glycerophosphocholines) in bronchoalveolar lavage fluid from air, vitamin E acetate (VEA), and cartridge-aerosol-exposed mice.
| Analyte | Air Mean |
± SEM | VEA Mean | ± SEM | p-Value | Significance | Cartridge Mean | ± SEM | p-Value | Significance |
|---|---|---|---|---|---|---|---|---|---|---|
| (vs. Air) | (vs. Air) | |||||||||
| DG(16:0_18:2_0:0) | 06.49 | ±0.20 | 05.05 | ±0.36 | <0.001 | *** | 04.38 | ±0.04 | <0.001 | *** |
| DG(16:0_16:0_0:0) | 00.19 | ±0.01 | 00.25 | ±0.04 | 0.5741 | NS | 00.40 | ±0.04 | 0.0031 | ** |
| CE(20:4) | 02.77 | ±0.10 | 02.52 | ±0.09 | 0.0002 | *** | 02.87 | ±0.24 | 0.2751 | NS |
| PC(16:0/16:0) | 29.86 | ±2.18 | 31.48 | ±2.41 | <0.001 | *** | 40.82 | ±2.64 | <0.001 | *** |
| PC(16:0_16:1) | 16.77 | ±1.42 | 17.59 | ±0.85 | 0.0295 | * | 23.46 | ±1.17 | <0.001 | *** |
| PC(16:0_18:2) | 04.31 | ±0.28 | 05.02 | ±0.48 | 0.0644 | NS | 06.74 | ±0.75 | <0.001 | *** |
| PC(16:0_18:1) | 04.63 | ±0.44 | 04.88 | ±0.17 | 0.6911 | NS | 06.64 | ±0.41 | <0.001 | *** |
| PC(14:0_16:0) | 04.22 | ±0.37 | 04.59 | ±0.34 | 0.4375 | NS | 05.79 | ±0.58 | <0.001 | *** |
| PC(16:0_22:6) | 01.86 | ±0.18 | 02.33 | ±0.32 | 0.2937 | NS | 03.18 | ±0.51 | <0.001 | *** |
| PG(16:0_18:1) | 02.70 | ±0.27 | 02.93 | ±0.11 | 0.7163 | NS | 03.85 | ±0.21 | 0.0014 | ** |
| PG(18:2_16:0) | 02.36 | ±0.16 | 02.84 | ±0.22 | 0.2688 | NS | 03.93 | ±0.37 | <0.001 | *** |
| PG(16:0/16:0) | 01.73 | ±0.14 | 01.85 | ±0.07 | 0.9170 | NS | 02.58 | ±0.12 | 0.0237 | * |
Normalized peak area means ± SEM with p-value and significance vs. air using two-way ANOVA (* p < 0.05, ** p < 0.01, and *** p < 0.001 vs. air, p > 0.05 are denoted as NS, n = 4/group).