Table 1.

Tools used for the in-silico characterization of hypothetical protein SCO0618

No.	Server name	Reference	Purpose
1	BLASTp	Johnson et al. (2008) [13]	Similarity search
2	protBLAST	Altschul et al. (1999) [40]
3	Muscle	Madeira et al. (2019) [14]	Multiple sequence alignment
4	Protparam	Gasteiger et al. (2005) [16]	Physicochemical characterization
5	Psortb	Yu et al. (2010) [18]
6	PSLpred	Bhasin et al. (2005) [19]	Subcellular localization prediction
7	Cello	Yu et al. (2006) [17]
8	SOSUIGramN	Imai et al. (2008) [20]
9	TMHMM	Moller et al. (2001) [21]	Topology prediction
10	HMMTOP	Tusnady and Simon (2001) [22]
11	CCTOP	Dobson et al. (2015) [23]
12	Motif	Kanehisa et al. (2002) [25]	Motif discovery
13	Pfam	Finn et al. (2015) [26]	Family relationship identification
14	Superfamily	Wilson et al. (2007) [27]	Superfamily search
15	COILS	Lupas et al. (1991) [28]	Coiled-coil motif identification
16	PFP-FunDSeqE	Shen and Chou (2009) [30]	Fold recognition
17	Interpro	Hunter et al. (2009) [29]	Functional classification
18	STRING	Szklarczyk et al. (2015) [31]	Interaction network analysis
19	PSIPRED	McGuffin et al. (2000) [32]	Secondary structure prediction
20	SOPMA	Geourjon and Deléage (1995) [33]
21	HHpred	Zimmermann et al. (2018) [34]	Tertiary structure prediction
22	PROCHECK	Laskowski et al. (1993) [36]
23	Verify3D		Structure verification
24	ERRAT