Figure 4.

Treg induction by staphylococcal protein A (SpA) in blocked in human serum. (A) Peripheral blood mononuclear cells (PBMC), (B) monocyte-derived dendritic cell (MoDC)/T cell cocultures, or (C) CD4⁺ T cells only were stimulated with SpA, SA113 WT or Sa Δspa for 5d in media containing chicken, human or no serum supplement (serum-free). CD3⁺CD4⁺CD25⁺CD127^dim cells were stained for viability and analyzed by flow cytometry. Results are shown from at least two independent experiments as single values of a minimum of four different donors with ± SD. The stars indicate statistical significance levels defined as *p < 0.05, **p < 0.01 and ***p < 0.001.