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. 2020 Sep 30;11:581713. doi: 10.3389/fimmu.2020.581713

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Copyright © 2020 Uebele, Habenicht, Ticha and Bekeredjian-Ding

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SA-induced, Treg-enriched supernatant has suppressive function. (A) Supernatant from monocyte-derived dendritic cells (MoDC)/T cell cocultures, stimulated with SA113 WT, was added to autologous CFSE-labeled CD4⁺ T cells. Upon stimulation with anti-CD3/CD28 beads, proliferation was analyzed by flow cytometry after 4d. Results are displayed as values from n = 6 single donors ± SD, analyzed in three independent experiments. Stars indicate statistical significance defined as *p < 0.05 and **p < 0.01. (B) CD4⁺ T cell proliferation upon stimulation with anti-CD3/CD28 beads ± Treg-enriched supernatant (SN) of one representative donor is shown as histogram.