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(A) The caspase-3 activity was determined. (B) Western blot analysis was performed to detect the expression level of pro-apoptotic Bcl-2 protein bax and anti-apoptotic protein bcl-2. β-actin was used as control protein. (C,D) Quantification analysis of bax and bcl-2. n=4. *P<0.05 vs. control ARPE-19 cells. #P<0.05 vs. ARPE-19 cells induced by H2O2 for 24 h.
